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. 2022 Aug 3;13:895095. doi: 10.3389/fendo.2022.895095

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Copyright © 2022 Feng, Ma, Li, Pei, Deng, Jia and Ma

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Mel inhibits Cyc-induced apoptosis of granulosa cells through mitochondrial apoptosis pathway. (A) Western blot analysis was performed on cleaved-caspase3, caspase3, Bax, and Bcl-2 in the ovaries of three-week-old ICR mice 12 h after Cyc (75 mg/kg) treatment with or without Mel (n = 6). ^a,b,c Bars with different letters are significantly different in each group (P < 0.05). (B) Western blot analysis was performed on mitochondrial Cyt-c (mito Cyt-c) and cytoplasm Cyt-c (cyto Cyt-c) in the ovaries of three-week-old ICR mice 12 h after Cyc (75 mg/kg) treatment with or without Mel (n = 6). *P < 0.05 compared with Sal group. ^# P < 0.05 compared with Cyc group.