Table 1.

Comparison of SenMayo with six existing senescence/SASP gene sets

	Human aging				Mouse aging								Mouse genetic clearance of senescent cells				Human pharmacological clearance of senescent cells
	Cohort A		Cohort B		Microglia		Prefrontal cortex		Dorsal hippocampus		Bone marrow		Mouse INK-ATTAC (old vs young)		Mouse INK-ATTAC (old, vehicle vs AP)		Adipose (Control vs D + Q)
	NES	p value	NES	p value	NES	p value	NES	p value	NES	p value	NES	p value	NES	p value	NES	p value	NES	p value
R-HSA-2559582	1.1075	0.2826	0.6780	0.9278	1.1794	0.1565	−1.2467	0.1169	1.2117	0.1916	0.9200	0.6043	1.4235	0.0326	1.0006	0.4442	1.1176	0.2356
Casella_up	1.0089	0.4748	0.6970	0.8885	0.9737	0.4790	0.9537	0.5209	1.3949	0.0593	0.8874	0.6339	−1.0861	0.3168	0.6865	0.9627	−0.7959	0.8344
Purcell	0.9329	0.5944	0.9585	0.5093	1.5120	0.0178	1.1731	0.2304	1.8117	0.0000	1.5224	0.0419	1.3894	0.0778	−0.7300	0.8874	0.9132	0.5852
Hernandez	0.7849	0.7771	0.7846	0.7802	0.7146	0.9461	0.8662	0.6710	−0.8100	0.7650	−0.5778	0.9789	1.2513	0.1718	1.4796	0.0266	0.9476	0.5266
Fridman_up	1.4249	0.0169	1.5407	0.0174	1.4397	0.0206	0.9639	0.5449	1.7482	0.0000	1.6100	0.0163	1.0762	0.3145	1.3413	0.0347	1.4187	0.0122
Sencan	−0.8460	0.9362	0.8038	0.8235	0.8674	0.8144	1.0328	0.4006	1.5302	0.0011	0.7247	0.8667	0.8838	0.7312	−1.0674	0.3182	0.8643	0.8983
SenMayo	1.5122	0.0023	1.4982	0.0031	1.4624	0.0052	1.6098	0.0013	1.8501	0.0000	1.5100	0.0362	1.5042	0.0023	1.4586	0.0054	1.4856	0.0020

Note that in GSEA analyses, p values < 0.25 are considered potentially significant, although we also identified p values <0.05 and <0.01 (NES, normalized Enrichment Score). Nominal p value, calculated as two-sided t-test, no adjustment since only one gene set was tested.