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. 2022 Aug 16;12:13908. doi: 10.1038/s41598-022-17730-x

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Pattern of H3K27me3 in 2-cell to 16-cell embryos. (a) Upper panel row shows the Z-projection of a whole embryo, counterstained with DAPI. Scale bar represents 20 µm. Bottom panels show a single section of a representative nucleus for each stage after staining with DAPI (green) and H3K27me3 (red). Last row is the merge of the two signals. Scale bar represents 5 µm for zoomed-in nuclei. (b) The graphs show the intensity profiles of DAPI (green) and H3K27me3 (red) signals across a single chromocenter highlighted by an arrowhead in (a). (c) Violin plots show the distribution of the Pearson’s Correlation Coefficients (PCC) between DAPI (green) and H3K27me3 (red) profiles at chromocenters for each stage (149 chromocenters analysed). Graphs were generated using ggplot2⁵⁶ (v3.3.5) in R and figures were arranged with FigureJ⁵⁵ in Fiji⁵⁴ (v1.53c).