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. 2022 Aug 16;12:13908. doi: 10.1038/s41598-022-17730-x

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Comparison of the pattern of H3K27me3 at chromocenters between embryonic to extra-embryonic lineages in peri-implantation stages embryos. (a, c and e) Upper left panel shows the Z-projection of a whole E3.5 (a), E4.0 (c) or E5.5 (e) embryo, counterstained with DAPI. Scale bar represents 20 µm. Upper right panels show a section with a highlight of ICM/EPI cells stained with NANOG (a, c) or OCT4 (c, e), TE or ExE cells stained with CDX2 (a, e) or PrE/VE cells stained with OCT4 only (c). Bottom panels show a representative nucleus for each presumptive lineage after immunostaining with H3K27me3 (red) and DAPI at E3.5 (a), E4.0 (c) and E5.5 (e). The last row is the merge of the two signals. EPI = Epiblast cells, PrE = Primitive Endoderm cells, TE = Trophoblast cells, ExE = Extra-embryonic Ectoderm cells, Emb-VE/Ex-VE = Visceral Endoderm cells in embryonic/extra-embryonic region respectively. (b, d and f) Graphs show the intensity profiles of DAPI (green) and H3K27me3 (red) signals across a single chromocenter highlighted by arrowheads in lower panels (a, c and e). Graphs were generated using ggplot2⁵⁶ (v3.3.5) in R and figures were arranged with FigureJ⁵⁵ in Fiji⁵⁴ (v1.53c).