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. 2022 Aug 16;12:13908. doi: 10.1038/s41598-022-17730-x

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Correlation dynamics between DAPI and H3K27me3 signals at chromocenters at blastocyst stages. (a, c and e) Pattern of H3K27me3 in a representative nucleus of EPI (a), PrE (c) and TE (e) cells at different blastocyst stages (from E3.25 to E4.25). DNA is stained with DAPI. Scale bar represents 5 µm. (b, d and f) Violin plots show the distribution dynamics of the Pearson’s Correlation Coefficients (PCC) between DAPI and H3K27me3 profiles at chromocenters in EPI (b), PrE (d) and TE (f) cells at various stages (262, 223 and 196 chromocenters analysed respectively). Graphs were generated using ggplot2⁵⁶ (v3.3.5) in R and figures were arranged with FigureJ⁵⁵ in Fiji⁵⁴ (v1.53c).