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. 2022 Aug 16;12:13908. doi: 10.1038/s41598-022-17730-x

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Transcription dynamics of major satellites in peri-implantation embryos assessed by RNA-FISH. (a, b) left panels show the Z-projection of whole embryos processed for immuno-RNA-FISH at E3.5 (a) and E4.0 (b). Scale bar represents 20 µm. EPI cells are either identified with NANOG staining (lower left panels, a and b) or morphologically (c). Lower right panels show zoomed-in nuclei of both embryonic or extra-embryonic lineages, with DAPI (blue) and major satellite (red) signals. Scale bar is 5 µm. (d) Proportion of cells in EPI or Extra-embryonic lineages exhibiting 0, 1–2, 3–4 and 5 or more (5 +) RNA-FISH foci per cell at the different indicated blastocyst stages (3400 nuclei analysed). Graphs were generated using ggplot2⁵⁶ (v3.3.5) in R and figures were arranged with FigureJ⁵⁵ in Fiji⁵⁴ (v1.53c).