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. 2022 Aug 16;12:13908. doi: 10.1038/s41598-022-17730-x

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Knock-down of EZH2 and its effects on H3K27me3 accumulation at PCH. (a, c) Early blastocysts at 96 h of culture after electroporation with scramble (a) or siEzh2 (c) and stained for EZH2 (grey), DAPI (green) and H3K27me3 (red). Upper panels show a z-projection of a whole embryo, while lower panels show a representative nucleus. (b, d) The graphs show the intensity profiles of DAPI, H3K27me3 and EZH2 signals across a single chromocenter. (e) Quantification of total signal intensity of EZH2 and H3K27me3 in nuclei (n = 20) of scramble, siEzh2 and siEzh2 + EPZ treated embryos. *** Pvalue < 10⁻³; ns = non significant; Mann–Whitney test. Graphs were generated using ggplot2⁵⁶ (v3.3.5) in R and figures were arranged with FigureJ⁵⁵ in Fiji⁵⁴ (v1.53c).