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. 2022 Aug 16;12:13908. doi: 10.1038/s41598-022-17730-x

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Comparative H3K27me3 enrichment at chromocenters during ESCs derivation and cEpiSCs conversion. (a) From left to right: H3K27me3 staining profile at different time points of ESC derivation from blastocysts cultured in 2i/LIF medium. (b) H3K9me3 staining in 72 h outgrowth. For (a) and (b), DAPI and NANOG staining are presented in the lower panels. The pluripotent cell population is delineated by a white dashed line. (c, e) ESCs and 10 days converted EpiSCs (e) colony stained for H3K27me3 (red), DAPI (green) and merge signals. (d, f) The graphs show the intensity profiles of DAPI, H3K27me3 and BEND3 signals across a single chromocenter for ESCs (d) and cEpiSCs (f). (g) Dynamics of H3K27me3 disappearance at 0 and 3 days post-medium switching from 2i/LIF to FGF2/Activin. Graphs were generated using ggplot2⁵⁶ (v3.3.5) in R and figures were arranged with FigureJ⁵⁵ in Fiji⁵⁴ (v1.53c).