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. 2022 May 21;127(4):637–648. doi: 10.1038/s41416-022-01835-1

Fig. 2. ALDH1L2 is a radiosensitive gene in vitro and in vivo.

Fig. 2

a Evaluation of protein expression by western blotting in seven colorectal cancer cell lines. β-actin served as a loading control. b Different ALDH1L2 transcript levels of seven CRC cells. c QRT-PCR for ALDH1L2 in HCT-15 and HCT 116 cell line after transfected with knockdown lentivirus. d QRT-PCR for ALDH1L2 in SW480 and HT-29 cell line after transfected with overexpression lentivirus. e Colony formation assay on different cell lines and its negative control. The survival curves of multi-target single-hit model on f HCT 116 cell lines and g SW480 cell lines. h, i Comet assay on different cell lines and its negative control. j Growth of tumour volume, k image and l weight of tumours harvested in nude mice injected with ALDH1L2-knockdown cell lines and its control, with/without 6 Gy irradiation. m Growth of tumour volume, n image and o weight of tumours harvested in nude mice injected with ALDH1L2 overexpressed cell lines and its control, with/without 12 Gy irradiation. p Immunofluorescence staining of Ki-67 in tumours from HCT 116 cell lines with ALDH1L2 knockdown and SW480 cell lines with ALDH1L2 overexpressed and their negative control, with irradiation or not. q Bar chart demonstrating the Ki-67 expression of HCT 116 cell lines. r Bar chart demonstrating the Ki-67 expression of the SW480 cell lines. *P < 0.05; **P < 0.01; ***P < 0.001.