Skip to main content
. 2022 Aug 16;13:4812. doi: 10.1038/s41467-022-32437-3

Fig. 5. PL and cholesterol efflux by WT ABCA1 and ABCA1 with mutations in the annulus and annulus orifice.

Fig. 5

WT and mutated ABCA1s (I74K/I371E, I74C/I371C, I371C/L375C, I371C, V304C/V308C) expressed in BHK cells were incubated for 4 h at 37 °C with APOA1 or an equal weight of the APOA1-mimetic peptide, L-4F. PL and cholesterol efflux, and total and cell-surface ABCA1 expression, were determined as described in Methods. Relative cholesterol and phospholipid efflux are normalized to WT and are presented as mean ± SD of three independent experiments with three replicates per experiment. Source data are provided as a Source Data file.