FIG. 2.

Plasmids used in this investigation. The DNA in each depicted insert is represented as a horizontal line extending between the restriction sites that mark the limits of the insert. The vectors pRK415 (39), pUC19 (66), and pBSK (Stratagene) have been described previously. All of the inserts were derived from the 14-kb EcoRI fragment in pZR135. The interruption in the horizontal line in pZR189 indicates the deletion produced by digestion with ClaI before recovery by gap repair of vanB chromosomal DNA in this plasmid. At the top of the figure are listed the functions tentatively assigned to open reading frames (indicated as open rectangles) on the basis of amino acid sequences similar to those of known proteins; the arrows indicate the directions of transcription. It must be emphasized that the some of the tentative functions assigned to proteins were shown to be incorrect as part of this investigation: a knockout mutation blocking the expression of the SalA-like protein did not prevent growth on salicylate, and the VanA-like protein did not complement mutations blocking the expression of vanA. Not depicted here are four additional BamHI sites (GGATCC) that were detected by sequencing the vanA-vanK intergenic region.