FIG. 3.

Characterized mutations in vanA and vanB. Linear bars represent vanA and vanB; expanded portions of the sequence depict the locations of mutations. Shaded rectangles indicate direct repetitions of chromosomal sequence flanking sites of insertion for mutations caused by insertion sequences or transposons. Horizontal arrows show the direction of transcription of the open reading frames within IS1236. Underlining marks the 7-bp direct chromosomal sequence repetition that appears to have directed the 13-bp vanA653 deletion. Characterization of DNA upstream from vanB674 was made possible by cloning of an EcoRI fragment containing DNA extending into the EcoRI site of Tn5613. Properties of DNA downstream from this site in vanB675 remains unknown.