TABLE 1.
Bacterial strains used in this investigation
| Strain | Parental strain | Genotype | Nature of mutation | Selection | Source or reference |
|---|---|---|---|---|---|
| ADP1 | BD413 | Wild type | 36 | ||
| ADP230 | ADP1 | pcaΔBDK1 | Engineered deletion | 30 | |
| ADP642 | ADP230 | pcaΔBDK1 ΔvanAB643 | Spontaneous deletion | Resistance to ferulate | This investigation |
| ADP643 | ADP642 | ΔvanAB643 | Growth with protocatechuate | This investigation | |
| ADP644 | ADP230 | pcaΔBDK1 vanA645 | Spontaneous insertion | Resistance to ferulate | This investigation |
| ADP645 | ADP644 | vanA645 | Growth with protocatechuate | This investigation | |
| ADP646 | ADP230 | pcaΔBDK1 vanB647::Tn5613 | Spontaneous insertion | Resistance to ferulate | This investigation |
| ADP647 | ADP646 | vanB647::Tn5613 | Growth with protocatechuate | This investigation | |
| ADP648 | ADP230 | pcaΔBDK1 vanB649 | Spontaneous bp substitution | Resistance to ferulate | This investigation |
| ADP649 | ADP648 | vanB649 | Growth with protocatechuate | This investigation | |
| ADP650 | ADP230 | pcaΔBDK1 ΔvanAB651 | Spontaneous deletion | Resistance to ferulate | This investigation |
| ADP651 | ADP650 | ΔvanAB651 | Growth with protocatechuate | This investigation | |
| ADP652 | ADP230 | pcaΔBDK1 ΔvanA653 | Spontaneous 13-bp deletion | Resistance to ferulate | This investigation |
| ADP653 | ADP652 | ΔvanA653 | Growth with protocatechuate | This investigation | |
| ADP654 | ADP230 | pcaΔBDK1 ΔvanAB655 | Spontaneous deletion | Resistance to ferulate | This investigation |
| ADP655 | ADP654 | ΔvanAB655 | Growth with protocatechuate | This investigation | |
| ADP656 | ADP230 | pcaΔBDK1 vanB657 | Spontaneous insertion | Resistance to ferulate | This investigation |
| ADP657 | ADP656 | vanB657 | Growth with protocatechuate | This investigation | |
| ADP672 | ADP230 | pcaΔBDK1 vanA673::Tn10′a | Mini-Tn10 | Resistance to both vanillate and kanamycin | This investigation |
| ADP673 | ADP672 | vanA673::Tn10′a | Growth with protocatechuate; loss of Kanamycin resistance | This investigation | |
| ADP674 | ADP230 | pcaΔBDK1 vanA675::Tn10′avanB675::Tn5613 | Mini-Tn10; spontaneous mutation | Resistance to both vanillate and kanamycin | This investigation |
| ADP675b | ADP672 | vanA675::Tn10′avanB675::Tn5613 | Growth with protocatechuate; resistance to kanamycin | This investigation |
a
The portion of Tn10 remaining in these strains is the 50-bp segment left by nearly precise excision (58).
b
This strain retains the mini-Tn10 kanamycin resistance marker, but it is not in the van genes.