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. 2022 Aug 3;12:933646. doi: 10.3389/fonc.2022.933646

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Copyright © 2022 Zhang, Tang, Guo, Wang, Zhou and Cai

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The antitumor responses of mice treated with nab-paclitaxel (nab-PTX) and anti-PD-1 antibody. (A) Macroscopic images and tumor growth curve in mouse LLC cancer models (n = 6, biological duplicates), statistically analyzed with two-way ANOVA followed by Tukey’s multiple comparisons. ***p< 0.001, ****p< 0.0001. (B) Immunohistochemistry staining of Ki67 for tumor sections (n = 3, magnification ×20, biological duplicates). The hematoxylin-stained nucleus is blue, and the positive expression of DAB is brownish yellow. (C) Apoptosis detection of tumor sections by TUNEL staining after the last administration (n = 3, magnification ×200, biological duplicates). Green, TUNEL staining; blue, DAPI. The red arrow denotes a representative TUNEL-positive cell.