Figure 8.
Effects of Serpinc1 gene on cell migration, invasion, proliferation, and apoptosis. The transfection of Serpinc1 was determined using (A) Western blot analysis and (B) qRT-PCR by one-way ANOVA (n = 3). (C) Wound healing assay was measured and analyzed using two-way ANOVA (n = 3, magnification ×200). The rates of (D) migration and (E) invasion were determined with transfection for 24, 48, and 72 h with two-way ANOVA (n = 3, magnification ×400). (F) Colony formation assay (n = 3) and (G) CCK8 assay (n = 5) of Serpinc1 overexpressing LLC cells were used to determine cell viability with ANOVA. (H) Apoptotic cells post-transfection were detected on flow cytometry using one-way ANOVA (n = 6). *p< 0.05, ***p< 0.001, ****p< 0.0001. Serpinc1-OE, Serpinc1 overexpression.