FIGURE 7.

Overexpression of p300 regulates the Wnt/β‐catenin pathway to inhibit IDD in rats. (a) LC3‐II/LC3‐I ratio in the IVD tissues of IDD rats as determined by Western blot assay. (b) Changes of protein expression of p300, FOXO3 and Sirt1 in the IVD tissues of IDD rats as determined by Western blot assay. (c) Changes of protein expression of Wnt/β‐catenin pathway‐related factors in the IVD tissues of IDD rats as determined by Western blot assay. (d) IDD in rats determined by the MRI method with Pfirrmann grading. (e) Autophagosomes in NPCs of rat IVD tissues as observed by TEM. (f) Safranin O‐fast green staining for the pathological changes of IVD tissues. (g) TUNEL staining for the apoptosis of rat IVD tissues (scale bar: 50 μm), green fluorescence represents TUNEL staining and blue fluorescence represents DAPI. The measurement data were expressed as mean ± standard deviation. * p < 0.05. ** p < 0.01. *** p < 0.001. **** p < 0.0001. Statistical comparison was conducted using one‐way ANOVA, followed by Tukey post‐hoc test. There were 6 rats in each group