TABLE 1.
Strains mutated in vanK
| Straina | Genotype
|
vanK mutation | VanK mutation | |
|---|---|---|---|---|
| pca | van | |||
| ADP7576 | ΔpcaK859 | Δvan1102 | ||
| ADP7577b | ΔpcaK859 | ΔvanK1103 | ΔG (366–371)d | |
| ADP7579 | ΔpcaK859 | Δvan1105 | ||
| ADP7580 | ΔpcaK859 | Δvan1106 | ||
| ADP7582 | ΔpcaK859 | ΔvanK1108 | ΔA (697–704)d | |
| ADP7584 | ΔpcaK859 | Δvan1110 | ||
| ADP7587 | ΔpcaK859 | ΔvanK1113 | G212A | Gly71Asp |
| ADP7592 | ΔpcaBDK1 | ΔvanK1103 | ΔG (366–371)d | |
| ADP7593 | ΔpcaBD1 | ΔvanK1103 | ΔG (366–371)d | |
| ADP7618 | ΔvanK1103 | ΔG (366–371)d | ||
| ADP7602c | ΔpcaK859 | ΔvanK7602 | Δ(A427–T522) | Δ(Ser143–Arg174) |
| ADP7603 | ΔpcaK859 | ΔvanK7603 | vanK::IS1236 | |
| ADP7604 | ΔpcaK859 | ΔvanK7604 | G460T | Gly154Trp |
| ADP7607 | ΔpcaK859 | ΔvanK7607 | Δ(T769–A918) | Δ(Cys257–Ala306) |
| ADP7608 | ΔpcaK859 | ΔvanK7608 | C655T | Gln219Stop |
| ADP7609 | ΔpcaK859 | ΔvanK7609 | G1133C | Arg378Pro |
| ADP7610 | ΔpcaK859 | ΔvanK1113 | G212A | Gly71Asp |
| ΔvanK7610 | T806C | Phe269Ser | ||
| ΔvanK7611 | T812C | Phe271Ser | ||
| ADP7612 | ΔpcaK859 | ΔvanK1113 | G212A | Gly71Asp |
| ΔvanK7612 | G880C | Gly294Arg | ||
a
ADP7576 to ADP7591 are derived from strains selected for a spontaneous mutation in pcaH or pcaG; ADP7602 to ADP7609 are derived from strains selected for a spontaneous mutation solely in vanK; ADP7610 and ADP7612 are derived from ADP7587 and contain PCR-generated suppressor mutations of vanK1113.
b
Same as ADP7578, ADP7581, ADP7583, ADP7585, ADP7586, ADP7589, and ADP7590.
c
Same as ADP7605, and ADP7606.
d
Frameshift mutation caused by deletion of one residue in a mononucleotide repeat.