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. 2022 Jul 18;21(8):e13673. doi: 10.1111/acel.13673

FIGURE 3.

FIGURE 3

PAM regulates extracellular matrix‐associated genes Timp2 and Vim. (a,b) ASCs (a) or C2C12 (b) were transfected with either control (siNC) or PAM siRNA (siPAM#1 or siPAM#2) oligos, the expression levels of PAM, Timp2 and Vim were detected 48 hours post‐transfection. (c) RT‐qPCR detection of PAM, Timp2 and Vim from C2C12 with or without PAM knockout. (d) ASCs (d) or C2C12 (e) were transfected with either control (siNC) or PAM siRNA (siPAM#1 or siPAM#2) oligos and chromatin conformation capture assay (3C‐qPCR) was performed to detect the chromatin interaction between PAM SE with Timp2 or Vim locus (f) the above 3C‐qPCR was performed in C2C12 cells with PAM knockout. (g,h) Genome browser tracks showing both knockdown (g) and knockout (h) of PAM led to reduction in H3K27ac signal intensity on Timp2 and Vim loci. Data information: Data represent the mean ± SD. p‐value was calculated by two‐tailed unpaired t‐test (*p < 0.05, **p < 0.01)