Table 1.
Strains and plasmids used in this study.
| Strain or plasmid | Description | Source |
|---|---|---|
| E. coli strains | ||
| DH5α | hsdR recA lacZYAF80 lacZΔM15 | BRL |
| S17-1 | F− recA hsdR RP4-2 (Tc::Mu) (Km::Tn7) lysogenized with λpir phage | Laboratory collection |
| Serratia marcescens strains | ||
| JNB5-1 | S. marcescens wild type strain | Pan et al. (2020) |
| SK6-35 | ompR::GmR mutant of JNB5-1, prodigiosin producing mutant | This study |
| SK6-35/pXW2010 | Mutant SK6-35 containing plasmid pXW2010 | This study |
| ΔOmpR | ompR deleted mutant of S. marcescens JNB5-1 | This study |
| ΔOmpR/pXW2010 | Mutant ΔOmpR containing plasmid pXW2010 | This study |
| PG-1 | Prodigiosin producing recombinant strain constructed by overexpression of psrA gene under the control of its own promoter PPsrA in strain JNB5-1 | This study |
| PG-2 | Prodigiosin producing recombinant strain constructed by overexpression of ompR gene under the control of its own promoter POmpR in strain JNB5-1 | This study |
| PG-3 | Prodigiosin producing recombinant strain constructed by overexpression of ompR and psrA genes under the control of their own promoters in strain JNB5-1 | This study |
| PG-4 | Prodigiosin producing recombinant strain constructed by overexpression of psrA gene under the control of the promoter P17 (PRplJ) in strain JNB5-1 | This study |
| PG-5 | Prodigiosin producing recombinant strain constructed by overexpression of ompR gene under the control of the promoter P17 (PRplJ) in strain JNB5-1 | This study |
| PG-6 | Prodigiosin producing recombinant strain constructed by overexpression both of ompR and psrA genes under the control of the promoter P17 (PRplJ) in strain JNB5-1 | This study |
| Plasmids | ||
| pRK2013Tn5G | Tn5G carrying plasmid, KmRGmR | Nunn and Lory (1992) |
| pMD18T | Cloning vector, 2,692 bp, ApR, lacZ | TaKaRa |
| pXW2010 | ompR gene driven by Plac promoter cloned in pUCP18, ApR | This study |
| pUCP18 | Broad-host-range shuttle vector, ApR | Schweizer (1991) |
| pUTKm | Tn5-based delivery plasmid with KmRAmpR | Herrero et al. (1990) |