TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Genotype | Source or reference |
|---|---|---|
| Strains | ||
| EK181 | (JLS9300) MC4100 rpoS::Tn10 | J. L. Slonczewski |
| EK198 | (GNB7145Km) MC4100 adiA::MudI1734 (Km lac) | G. N. Bennett |
| EK221 | (MDA7K) MC4100 cysB trpB::Tn10 | G. N. Bennett |
| EK227 | E. coli K-12 wild type | AC Matin |
| EK239 | (JLS9391) MC4100 gadC::Tn10 | 11 |
| EK293 | S17-1 λpir | 5 |
| EK345 | (G1297) Δcya::Km Δcrp::Cm | S. Garges |
| EF333 | K12 gadC::Tn10 | EK239 × EK227 |
| EF337 | K12 adiA::MudI1734 (Km lac) gadC::Tn10 | EK198 × EF333 |
| EF362 | K12 rpoS::Tn10 | EK181 × EK227 |
| EF393 | K12 rpsL | EK227, spontaneous Strr |
| EF438 | K12 adiA::pKnock-Ap | |
| EF468 | EK293/pCF305 (gadC internal fragment in pRR10-250v) | This study |
| EF471 | EK293/pCF312 (gadA internal fragment in pRR10-250v) | This study |
| EF490 | K12 rpsL gadC::pRR10 (Ap) | EF393 × EF468 |
| EF491 | K12 gadC::pRR10 (Ap) | EK227 × EF490 |
| EF492 | K12 rpsL gadA::pRR10 (Ap) | EF393 × EF471 |
| EF493 | K12 gadA::pRR10 (Ap) | EK227 × EF492 |
| EF507 | K12 gadB::Km | EK227 × pCF325 |
| EF522 | K12 gadA::pRR10 (Ap) gadB::Km | EF493 × EF507 |
| EF529 | K12 Δcrp::Cm | EK345 × EK227 |
| EF530 | K12 Δcya::Km | EK345 × EK227 |
| EF531 | K12 gadA::pRR10 (Ap) rpoS::Tn10 | EF493 × EF362 |
| EF532 | K12 gadB::Km rpoS::Tn10 | EF507 × EF362 |
| EF547 | K12 gadC::pRR10 (Ap)/pCF348 (gadC+ Tcr) | EF491 × pCF348 |
| EF548 | K12 gadA::pRR10 gadB::Km/pCF348 (gadC+ Tcr) | EF522 × pCF348 |
| EF549 | K12 gadB::Km/pCF348 (gadC+ Tcr) | EF507 × pCF348 |
| Plasmids | ||
| pCR2.1 | Invitrogen | |
| pSK− | Stratagene | |
| pRR10-250v | 10, 19 | |
| pMAK705 | 10 | |
| pHP45ΩKm | 7 | |
| pHP45ΩTet | 7 | |
| pFP256 | pCR2.1 containing gadC+ controlled by lacP | |
| pCF301 | pSK− with internal fragment (oligonucleotides 105 and 106) of gadC | |
| pCF303 | pSK− with internal fragment (oligonucleotides 107 and 109) of gadA | |
| pCF305 | pRR10-250v (EcoRI-HindIII) with gadC (pCF301, EcoRI-HindIII) | |
| pCF312 | pRR10-250v (EcoRI-HindIII) with gadA (pCF303, EcoRI-HindIII) | |
| pCF319 | pCR2.1 with gadBC′ fragment (oligonucleotides 121 and 106) | |
| pCF324 | pMAK705 (XbaI-HindIII) with gadBC′ fragment (XbaI-HindIII) from pCF319 | |
| pCF325 | pCF324 gadBC′::Km | |
| pCF348 | pFP256 with Tet from pHP45ΩTet (gadC+ KmsTcr) |