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Glutamate-dependent AR in log-phase and stationary-phase cells
| Growth phase | % Survival after adaptation ina:
|
||
|---|---|---|---|
| EG (pH 5.5) | E medium plus glycerol (pH 5.5) | LBG | |
| Exponential | 0.07 | NDb | 0.08 |
| Stationary | 70 | 74 | 76 |
EK227 was grown to exponential phase (OD600 = 0.5) or stationary phase (overnight or for 22 h) in the media indicated. Glycerol was added at 0.4%. Cells were diluted 1:1,000 into EG containing 1.5 mM glutamate (pH 2.5). Survival was measured after 4 h.
ND, not determined.
