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Adaptation in BHI and effects of gad and adiA mutations on AR at pH 2
| Strain | Pertinent genotype | % Survival after adaptationa
|
|
|---|---|---|---|
| BHI | BHIG | ||
| EK227 | Wild type | 20 | 65 |
| EK198 | adiA | 70 | 55 |
| EF333 | gadC | 0.003 | 0.8 |
| EF337 | adiA gadC | 0.002 | 0.003 |
Cells were grown to stationary phase in the media indicated. The cells were diluted to 1 × 106 to 3 × 106 CFU/ml in acid challenge media (pH 2 LB). The results presented indicate survival after 1 h.
