Figure 4.

Characterization and organization of cells inside the scaffold

(A) number of cells (mean ± SD) usually retrieved from the scaffold after 48 h or 72 h of 3D culture in bioreactor.

(B) Characterization by flow cytometry of HS-5 cells, MEC-1 cells and MM1.S cells.

(C) Characterization of scaffolds populated with HS-5 BMSCs and MEC-1 (LEFT) or MM.1S (RIGHT).

(D) Confocal analysis of scaffold populated with HS-5 BMSC line (bar size=50 μm; Leica TC sp5).

(E) Confocal analysis of scaffold populated with HS-5 BMSC line and CLL cell line MEC (bar size=50 μm; Leica TC sp5).

(F) Confocal analysis of scaffold populated with HS-5 BMSC line and MM cell line MM.1S (bar size=50 μm; Olympus Fluo view).