Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
| Complete tissue culture medium (CTM) | Final concentration | Amount/volume |
|---|---|---|
| RPMI1640 with L-glutamine or DMEM with 4.5 g/L glucose | 89% | 445 mL |
| Heat Inactivated FBS | 10% | 50 mL |
| Penicillin/Streptomycin solution | 1% | 5 mL |
| Total | 100% | 500 mL |
Storage notes: Prepare CTM under sterile conditions using a biosafety cabinet, store at 4°C and pre-warm at 37°C before use. CTM can be stored at 4°C for 1 month.
