Velcade® | Final concentration | Amount/volume |
---|---|---|
1st dilution (solution A) | ||
Velcade® (6.5 mM) | 6.5 μM | 1 μL |
CTM | N/A | 1 mL |
2nd dilution | ||
Solution A | 10 nM | 16.9 μL |
CTM | N/A | 11 |
Total | ∼11 mL |
Storage note: The proteasome inhibitor Bortezomib (Velcade®, Millennium Pharmaceuticals, Inc., Cambridge, MA) is stored at −20°C and diluted in CTM immediately before use.