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. 2022 Aug 10;3(3):101601. doi: 10.1016/j.xpro.2022.101601
Velcade® Final concentration Amount/volume
1st dilution (solution A)
Velcade® (6.5 mM) 6.5 μM 1 μL
CTM N/A 1 mL
2nd dilution
Solution A 10 nM 16.9 μL
CTM N/A 11
Total ∼11 mL

Storage note: The proteasome inhibitor Bortezomib (Velcade®, Millennium Pharmaceuticals, Inc., Cambridge, MA) is stored at −20°C and diluted in CTM immediately before use.