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. 2022 Aug 10;3(3):101601. doi: 10.1016/j.xpro.2022.101601
REAGENT or RESOURCE SOURCE IDENTIFIER
Antibodies
APC, anti-human CD73, REAfinity™ (REA804) Miltenyi Biotec Cat# 130-111-909 RRID: AB_2659167
PC7, anti-human CD19 (J3-119) Beckman Coulter Cat# IM3628 RRID: AB_10638575
PE, anti-human CD38 (HIT2) Thermo Fisher Scientific Cat# MHCD3804 RRID: AB_1472940
PC7, anti-human CD38 (HIT2) BD Biosciences Cat# 560677 RRID: AB_1727473
FITC, anti-human CD138 (MI15) BD Biosciences Cat# 552723 RRID: AB_394443
Chemicals, peptides, and recombinant proteins
Phalloidin-ATTO 565 MERCK, Sigma Aldrich Cat# 94072
Hoechst 33342, Trihydrochloride, Trihydrate - 10 mg/mL Solution in Water Thermo Fisher Scientific Cat# H3570
Trypan blue solution Merck, Sigma-Aldrich Cat# T8154
Triton ™ X100 Merck, Sigma-Aldrich Cat# T9284
RPMI 1640 with L-glutamine Euroclone S.p.A Cat# ECB2000
DMEM with L-glutamine and High Glucose Euroclone S.p.A Cat# ECM0103L
Fetal Bovine Serum (FBS) Euroclone S.p.A Cat# ECS5000L
Penicillin-Streptomycin Solution 100× Euroclone S.p.A Cat#ECB3001D
Dulbecco’s PBS w/o Calcium & Magnesium (PBS) Euroclone S.p.A Cat# ECB4004L
PBS with Ca++/Mg++ Merck, Sigma-Aldrich Cat# D8662-500ML
Trypsin–EDTA 1× solution (0.5 g/L Trypsin-0.021 g/L EDTA Euroclone S.p.A Cat# ECM0920D
LiberaseTM TM Research Grade Roche, Merck Cat# 5401119001
Paraformaldehyde (PFA) 4% of your choice N/A
Ibrutinib Selleck Chemicals Cat# PCI-32765
Bortezomib (Btz) (Velcade®) Millennium Pharmaceuticals N/A
Bovine Serum Albumin (BSA) Merck, Sigma-Aldrich Cat# A7906
cOmpleteTM: protease inhibitor cocktail Roche, Merck Cat# 11697498001
NP40 cell lysis buffer Thermo Fisher Scientific Cat# FNN0021
TRIzol® Reagent Ambion®, Inc by Life Technologies Cat# 15596018
Critical commercial assays
AnnexinV-FITC/Propidium Iodide (PI) Apoptosis Detection kit Thermo Fisher Scientific Cat# BMS500FI-300
Vybrant® CFDA SE Cell tracer Kit Thermo Fisher Scientific Cat# V12883
Experimental models: Cell lines
HS-5: human HPV-16 E6/E7 transformed BMSCs American Type Cell Culture (ATCC) Cat# CRL-11882
MEC-1 cells DSMZ Cat# ACC497
MM1.S cells American Type Cell Culture (ATCC) Cat# CRL-2974
Software and algorithms
FCS Express Flow Cytometry software De Novo Software https://denovosoftware.com/
Other
Gelatin sponge, SpongostanTM Ethicon, Inc. USA Cat# MS0005/J&J
Rotary Cell Culture System (RCCSTM) bioreactor Synthecon, Inc Cat# RCCS-4DQ https://synthecon.com/pages/disposable_vessel_system_rccs4dq_synthecon_20.asp
4 Pack individually wrapped gamma sterilized disposable 10 mL vessels (High Aspect Ratio Vessel-HARV) Synthecon, Inc, USA Cat# D-410
96-well Suspension Culture Plate, U bottom Greiner Bio-One, CELLSTAR Cat# 650185
Forceps of your choice N/A
Biopsy punch (4 mm diameter) of your choice N/A
Sterile scalpels of your choice N/A
5 mL syringes of your choice N/A
2 mL syringes of your choice N/A
1 mL syringes of your choice N/A
Micro-pipette of your choice N/A
Tips for micropipette of your choice N/A
Disposable plastic-ware for cell culture of your choice N/A
6-well plate of your choice N/A
48-well plate of your choice N/A
Confocal Microscope of your choice N/A
Multi-color Flow cytometer of your choice N/A
Sterile class II biosafety cabinet of your choice N/A
Incubator with humidified atmosphere, at 37°C and 5% CO2 of your choice N/A
Refrigerated centrifuge of your choice N/A
Hemocytometer, Burker chamber of your choice N/A
Water bath with controlled temperature of your choice N/A
FACS tubes with 35 μm cell strainer caps Falcon Cat##352235