Figure 3.

Temporal regulation of systemic metabolism is coordinated by light and tryptophan. A-B. Venn diagram representing the overlap of temporally regulated (comparing ZT4 and 16) metabolites between control (Ctrl) and tryptophan depletion (-Trp) in the SCN, serum and the liver. Mice were housed under 12-h light/dark cycles (A) or constant darkness (B). C-D. Log₂ fold change (ZT16/ZT4) of the overlapping metabolites under 12-h light/dark cycles (C) or constant darkness (D). E-F. Circos plots represent correlations of metabolites between tissues from mice housed under light/dark cycles (E) or constant darkness (F). Red connections represent positive correlations and blue connections represent negative correlations. G. Bar chart showing the number of significantly correlating metabolites between the tissues and the serum in each group. H. Phenyllactate levels in the SCN, serum and liver at two time points illustrated as bar charts. Significant differences between time points were determined using a Two-Way ANOVA. Significant differences in the temporal fold change between dietary interventions were determined by first converting all negative values to positive followed by a paired students t-test of the Log₂ fold changes and Bonferroni correction. N = 5 mice. NS = not significant. ∗P < 0.05 and ∗∗P < 0.01. Data are mean ± s.e.