Figure 6.

Dietary tryptophan controls the hepatic core clock machinery in a light-dependent manner. A-B. Gene expression of core clock genes in the liver of mice housed under light/dark cycles (A) or constant darkness (B). C. Representative western blots of hepatic core clock proteins. The western blot analyses were repeated in n = 4 and the quantification is illustrated in the graphs. D. Gene expression of selected circadian genes from mice from the experiment in Figure 1F. Livers were harvested at CT4 after the last injection (CT10-11 the day before). One-way ANOVA was used to determine statistically significant differences between more than two groups and multiple comparisons were subsequently analyzed with Fischer's least significant difference test. ∗P < 0.05, ∗∗P < 0.01 and ∗∗∗P < 0.001. Data are mean ± s.e.