Figure 4.
PODXL isoforms regulate cell sensitivity to cisplatin to different degrees
(A) Cytotoxic index values of U2OS WT cells, U2OS cells overexpressing empty (control) backbone, the PODXL isoforms, scrambled control shRNA (shctrl), or PODXL shRNAs (shPODXL1, shPODXL2) were generated (See also Figure S4). Cells were treated with 30 μM cisplatin for 48 h. Cytotoxic index was calculated by normalizing the dead cell object counts against the total number of DNA-containing object counts. Three independent sets of experiments were performed with three biological replicates included in each experiment. Data are plotted as mean ± SEM. The p-values were calculated using Student’s t-test (∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001).
(B) Dose-response curves for the U2OS cells with PODXL overexpression or KD, and controls (WT, Empty, shctrl). The plot shows one set of experiment performed with two biological replicates. Data are plotted as mean ± SEM.
(C) EC50 of U2OS cells with PODXL overexpression or KD, and control cell lines. Three independent sets of experiments were performed with two biological replicates included in each experiment. Data are plotted as mean ± SEM. The p-values were calculated using Student’s t-test (∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001).
(D) Apoptotic Index values of U2OS cells with PODXL KD. The assay was ended at 26 h after cisplatin treatment (30 μM). Two independent sets of experiments were performed with three biological replicates included in each experiment. Data are plotted as mean ± SEM. The p-values were calculated using Student’s t-test (∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001).
(E) Similar to (D), for PODXL OE cells. The assay was ended at 40 h after cisplatin treatment (30 μM). Data are plotted as mean ± SEM. The p-values were calculated using Student’s t-test (∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001).