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. 1999 Jun;181(11):3552–3561. doi: 10.1128/jb.181.11.3552-3561.1999

FIG. 7.

FIG. 7

Binding of ς32 mutant proteins to RNAP. 3H-labeled ς32 proteins (wild type [WT], ς32-F137E, and ς32-I123D) (A) were incubated with RNAP, and a 30-fold excess of wild-type unlabeled ς32 (B and C, left) or a 10-fold excess of unlabeled ς70 (C, right) was added. At the indicated times, samples were subjected to gel filtration, and the amount of labeled protein was quantified and is expressed as the percentage of the total labeled protein (B) or as a relative amount of RNAP-bound ς32 recovered at time zero after the addition of competitor (C). (B) Open circles, 0-min chase with competitor; solid squares, 5-min chase; solid triangles, 60-min chase. (C) Open squares, ς32-F137E; solid circles, wild-type ς32; solid triangles, ς32-I123D.