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. 2022 Apr 9;107(2):458–473. doi: 10.1093/biolre/ioac070

Figure 4.

Figure 4

The effects of SMAD3 knockdown on the activin A-induced upregulation of HAS2 and versican in SVOG cells. (A and B) SVOG cells (n = 3) were transfected with 50 nM siCtrl or 50 nM siRNA targeting SMAD3 (siSMAD3) for 48 h and then the cells were treated with Ctrl or 25 ng/mL activin A for an additional 12 h. The mRNA (A) and protein (B) levels of SMAD3 were examined using RT-qPCR and western blotting, respectively. (C and D) SVOG cells (n = 3) were transfected with 50 nM siCtrl or 50 nM siSMAD3 for 48 h and then the cells were treated with Ctrl or 25 ng/mL activin A for an additional 12 h. The mRNA (C) and protein (D) levels of HAS2 were examined using RT-qPCR and western blotting, respectively. (E and F) SVOG cells (n = 3) were transfected with 50 nM siCtrl or 50 nM siSMAD3 for 48 h and then the cells were treated with Ctrl or 25 ng/mL activin A for an additional 12 h. The mRNA (E) and protein (F) levels of versican were examined using RT-qPCR and western blotting, respectively. The results are expressed as the means ± SEM. Values without a common letter were significantly different (P < 0.05).