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. 2022 Apr 9;107(2):458–473. doi: 10.1093/biolre/ioac070

Figure 7.

Figure 7

The effects of the type I receptor inhibitor SB431542 on the activin A-induced increase in hyaluronan accumulation in SVOG and primary hGL cells. (A and C) SVOG (A) and primary hGL (C) cells were treated with 25 ng/mL and 50 ng/mL activin A for 12 h, respectively. The accumulated levels of hyaluronan in conditioned medium were measured using ELISA. (B and D) SVOG (B) and primary hGL (D) cells were pretreated with DMSO or SB431542 (10 μM) for 30 min, and the cells were treated with Ctrl or 25 ng/mL (SOVG hGL) and 50 ng/mL (primary hGL) activin A for an additional 12 h. The accumulated levels of hyaluronan in conditioned media were examined using ELISA. The results are expressed as the means ± SEM of at least three independent experiments. Student’s t-test was used to analyze two groups (*P < 0.05). One-way ANOVA was used to analyze multiple groups (values without a common letter were significantly different, P < 0.05).