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. 2022 Apr 9;107(2):458–473. doi: 10.1093/biolre/ioac070

Figure 8.

Figure 8

The effects of ALK4, ALK5, or ALK7 knockdown on the activin A-induced upregulation of HAS2 and versican in primary hGL cells. (A and B) SVOG cells (n = 3) were transfected with 50 nM siCtrl, 50 nM siRNA targeting ALK4 (siALK4), 50 nM siRNA targeting ALK5 (siALK5), or 50 nM siRNA targeting ALK7 (siALK7) for 48 h and then the cells were treated with Ctrl or 25 ng/mL activin A for an additional 12 h. The mRNA (A) and protein (B) levels of HAS2 were examined using RT-qPCR and western blotting, respectively. (C and D) SVOG cells (n = 3) were transfected with 50 nM siCtrl, 50 nM siALK4, 50 nM siALK5, or 50 nM siALK7 for 48 h and then the cells were treated with Ctrl or 25 ng/mL activin A for an additional 12 h. The mRNA (C) and protein (D) levels of versican were examined using RT-qPCR and western blotting, respectively. The results are expressed as the means ± SEM. Values without a common letter were significantly different (P < 0.05).