Table 2.
Primer sequences for qRT-PCR analysis in Nile tilapia.
| Gene name | Sense and antisense primer (5′–3′) | GenBank no. |
|---|---|---|
| ef1α | F: CTACGTGACCATCATTGATGCC R: AACACCAGCAGCAACGATCA |
AB075952 |
| β-actin | F: AGCCTTCCTTCCTTGGTATGGAAT R: TGTTGGCGTACAGGTCCTTACG |
KJ126772 |
| pparα | F: CTGATAAAGCTTCGGGCTTCCA R: CGCTCACACTTATCATACTCCAGCT |
KF871430 |
| cpt1b | F: AAGGGACGTTACTTCAAGGTG R: TCCGACTTGTCTGCCAAGAT |
GQ395696 |
| accβ | F: ACATGCAGTCCATGCTGCGT R: AAATGCCTCTCAAGCCACTCAA |
XM_003451659 |
| srebp1 | F: TGCAGCAGAGAGACTGTATCCGA R: ACTGCCCTGAATGTGTTCAGACA |
XM_005457771 |
| accα | F: TAGCTGAAGAGGAGGGTGCAAGA R: AACCTCTGGATTGGCTTGAACA |
XM_005471970 |
| fas | F: TTGAGGATGTGACTATCCACAGGG R: GTCAGGTTTCCGTTCTCCGAAA |
XM_003454056 |
| dgat | F: GCTTGAATTCTGTCACCCTGAAGA R: ACCTGCTTGTAGGCGTCGTTCT |
XM_003458972 |
| mtco1 | F: CTGTTTATCCCCCACTCGCA R: AATAGATGACACCCCGGCCA |
LC189956.1 |
| sdha | F: GGTATTCCGTACCGGCTCTG R: GTCGGTGTTCCACACAATGC |
XM_003443687.5 |
| ndufa9 | F: ACCTTTTGTGCCCTACCCTC R: TTTGTCTGGGGTTGTCCAGG |
XM_003447056.4 |
| gk | F: GACATGAGGACATTGACAAGGGAA R: CTTGATGGCGTCTCTGAGTAAACC |
XM_003451020.2 |
| pfk | F: AACCTGTGTGTGATTGGAGGTGAT R: CGTGATCTTACCGGCTTTAACAAG |
XM_003441476.2 |
| pk | F: CAGCATAATCTGCACCATCGGT R: ATGAGAGAAGTTAAGACGGGCGA |
XM_005472621.3 |
| pepck | F: TGGAAGAACAAACCTTGGCG R: TGGGTCAATAATGGGACACTGTCT |
XM_003448375 |
| g6pase | F: AGACCTTATTGGTGGGTTCACGA R: CTGAAGGACTTCCTGGTCCAGTTT |
XM_003448671.4 |
| fbpase | F: ACCGGACAATAGCGGAAAATACA R: TGGCGAATATTGTTCCTATGGAGA |
XM_003449650.4 |
| idh | F: ACGCATCGCTGAGTACGCCTT R: AGACCGTCTGACATCCGCATGA |
XM_003437590.5 |
| cs | F: AGCACCACAGTTTACCAG R: AGTGTTGACAAACCCAGA |
XM_003438897 |
| glut2 | F: CATTGGCATTCTAATCAGCCAGGT R: TTGTAATATTGCTGGCGCTCCA |
XM_003442884.5 |
| glut4 | F: GCAGGAGGAAAGCCATGCTTATA R: ATCATTTCAAAGGAGCGGCAGA |
XM_003458705.4 |
| gs | F: CCTCACTCTGCGCTGTTATTC R: CAGCGGCATGCCTTCAGTTT |
XM_013276796.3 |
| pdk1 | F: GAGGAGCAGCGTGTCCATAG R: AGGTAACTCCTGTCAAAATCCAGA |
XM_003447311.5 |
| pdk2 | F: GCAGAGTTCATCCAGACAA R: GACCTGTAGTGCTTATCTGAT |
XM_003448725.5 |
| pdk3 | F: GTCATGTCATTGCGAAGGGC R: CAGAGCCAGCTCCATAAGGTT |
XM_005471769.4 |
| pdk4 | F: AATCCACAGCCAGTCACT R: GCAGAGTTCATCCAGACAA |
XM_003457260.5 |
| pdhe1α | F: AATCCACAGCCAGTCACT R: GCAGAGTTCATCCAGACAA |
XM_013264731.3 |
ef1α = elongation factor 1 alpha; pparα = peroxisome proliferator activated receptor α; cpt1b = carnitine palmitoyl transferase 1b; accβ = acetyl-coa carboxylase β; srebp1 = sterol regulatory element binding transcription factor 1; accα = acetyl-coa carboxylase α; fas = fatty acid synthase; dgat = diacylglycerol o-acyltransferase; mtco1 = mitochondrial cytochrome c oxidase 1; sdha = succinate dehydrogenase complex subunit A; ndufa9 = NADH dehydrogenase [ubiquinone] 1a subcomplex subunit 9; gk = glucokinase; pfk = phosphofructokinase; pk = pyruvate kinase; pepck = phosphoenolpyruvate carboxykinase; g6pase = glucose-6-phosphatase; fbpase = fructose-1,6-bisphosphatase; idh = isocitrate dehydrogenase; cs = citrate synthase; glut2 = glucose transporter 2; glut4 = glucose transporter 4; gs = glycogen synthase; pdk1/2/3/4 = pyruvate dehydrogenase kinase 1/2/3/4; pdhe1α = pyruvate dehydrogenase E1α subunit.