Figure 5.

Anc80L65-Eps8 rescue of mechanoelectrical transduction in Eps8^−/− mice

(A–C) Saturating MET currents in apical P9 IHCs from control Eps8^+/+ (A), Eps8^−/− (B), and Eps8^−/− injected with Anc80L65-Eps8 (C) mice in response to 50-Hz sinusoidal force stimuli to the hair bundles at membrane potentials of −124 and +96 mV. Driver voltage (DV) stimuli to the fluid jet are shown above the traces, with positive deflections of the DV being excitatory. The arrows and arrowheads indicate the closure of the transducer channel in response to inhibitory bundle stimuli at −124 and +96 mV, respectively. (D) Peak-to-peak MET current-voltage curves obtained from P9 IHCs of the mouse models described in (A)–(C). Recordings were obtained by mechanically stimulating the hair bundles of IHCs while stepping their membrane potential from −124 to +96 mV in 20-mV increments. (E and F) Maximal size of the MET current measured at −124 mV (E) and +96 mV (F) from Eps8^+/+, Eps8^−/−, and Eps8^−/− injected with Anc80L65-Eps8. One-way ANOVA: p = 0.0031 (E) and p = 0.0057 (F) is indicated by the star symbol. For post test, see section “results”. (G and H) Resting open probability (P_o) of the MET current in IHCs from the three above mouse models at the holding of −124 mV (G) and +96 mV (H). The resting current is given by the holding current minus the current present during inhibitory bundle deflection. One-way ANOVA: p = 0.0002 (G) and p = 0.0138 (H) is indicated by the star symbol. For post test, see section “results”. Data are plotted as means ± SD.