Figure 8.

Hair bundles in IHCs transduced with Anc80L65-Eps8 in the adult cochlea

(A) Confocal images of the hair bundles from IHCs of 3-month-old Eps8^−/− mice transduced with Anc80L65-EPS8 at P20. Adult cochleae were immunostained for EPS8 (red) and stereocilia are labeled with phalloidin (green). Note that transduced IHCs still show several rows of stereocilia, most of which remained short. Moreover, EPS8 is no longer localized at the tip of the taller stereocilia. (B–E) Confocal images using the same experimental conditions described in (A), but using antibodies against MYO15 (B and C) and WHIRLIN (D and E), in addition to EPS8. Note that the diffuse staining of all the proteins, similar to the labeling seen in basal-coil IHCs from Eps8^−/− mice transduced with Anc80L65-EPS8 at P1–P2. Scale bar in the inset of (E), which also applies for (C), represents 1 μm.