Table 1.
Details of SNP evaluated in the study with early primary knee osteoarthritis and controls
| S. No | SNPedia | Orientation Details |
| 1 | Gene | MTHFR |
| 2 | Reference sequence number | rs1801133 |
| 3 | Condition | Early primary knee Osteoarthritis |
| 4 | Organism | Homo sapiens |
| 5 | Mutation | Non-synonymous single nucleotide polymorphism |
| 6 | Amnio acid substitution | C677T (Ala 677 Val) |
| 7 | Single Nucleotide Polymorphism | C-T |
| 8 | Exon position | Exon 5 |
| 9 | Chromosome Region | Chromosome-1 p36.22 |
| 10 | 5′-3′ Primer sequence | TGAAGGAGAAGGTGTCTGCGGGA |
| 11 | 3′-5′ Primer Sequence | GGACGGTGCGGTGAGAGTG |
| 12 | PCR Amplicon size | 198bp |
| 13 | Restriction Enzyme | Hinf1 |
| 14 | Substitution of Nucleotide band size | 176bp |
| 15 | Digested amplicon size | CC: 198bp ; CT:198/176/282bp ; TT176/22bp |
| 16 | Genotype effect | CC: Normal or no risk CT: Increased risk TT: Increased risk |