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. 2022 Aug 17;12:13943. doi: 10.1038/s41598-022-14730-9

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Derivative melt curve released of approach one, related to HPV 16 as a diagnostic standard and isolate 4BA as microarray-identification HPV 53 (a). Derivative melt curve released of approach two, related to HPV 16 as a diagnostic standard and isolate 4BA as microarray-identification HPV 53 (b). Schematic map of the amplification region of human papillomaviruses 16 and 53 in this study and the location of the primers using CLC workbench 12 software (c). Pairwise comparison of the amplified region in approach one related to HPV 16 (diagnostic standard) and isolate 4BA (HPV 53) using CLC workbench 12 software (d).