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. 2022 Aug 17;12:13943. doi: 10.1038/s41598-022-14730-9

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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The derivative melt curve released of approach one is related to human papillomaviruses of isolates G11, G42, and G40 (a). The derivative melt curve released from approach two is related to isolates G11, G42, and G40 (b). Schematic map of the sequencing result of amplification region G11 and the presence of HPV mix infection after editing the sequence using CLC workbench 12 software (c). Schematic map of the sequencing result of amplification region G42 and existence of HPV mix infection after editing the sequence using CLC workbench 12 software (d).