Table 3.
List of prioritized MHC-I (CTL) binding epitopes by NetCTL 1.2 server.
| Protein | MHC-I Epitopes | No. of HLASuper-types | HLA supertypes (IC50 nM) | Total HLAscore | AT | Allergenicity | Toxicity | Immunogenicity |
|---|---|---|---|---|---|---|---|---|
| E | FTVVCAITL | 4 | A2 (380.29), B8 (135), B39 (245.13), B62 (95.11) | 3.628 | 1.05 | NA | NT | 0.17427 |
| M | GTNSGVAIY | 4 | A1 (77.11), A3 (259), A26 (133), B62 (142.97) | 5.428 | 0.46 | NA | NT | 0.01407 |
| ALSIFSAVY | 4 | A1 (282), A3 (68.46), B58 (101.2), B62 (170.70) | 4.529 | 0. 61 | NA | NT | 0.09563 | |
| YPSRSMTVY | 5 | A1 (246.49), A26 (273.6), B7 (529.53), B8, B62 (66.87) | 4.734 | 0. 92 | NA | NT | 0.27114 | |
| LLITIVLQY | 5 | A1 (126.26), A3 (580.50), A26 (111.73), B58 (70.81), B62 (567.85) | 5.823 | 0.87 | NA | NT | 0.17368 | |
| N | STPAQNAGY | 5 | A1 (122.99), A3 (874.13), A26 (187.76), B58 (811.5), B62 (98.00) | 7.243 | 0.44 | NA | NT | 0.0303 |
| SAFMGMSQF | 5 | A26 (189.00), B7 (351.4), B8 (669.1), B58 (408.43), B62 (27.24) | 3.793 | 0.44 | NA | NT | 0.4783 | |
| S | WSYTGSSFY | 5 | A1 (207), A3 (405.32), A26 (128.17), B58 (415.24), B62 (86.49) | 6.884 | 1.06 | NA | NT | 0.15609 |
| YSTNITHLL | 5 | A1 (96.5), A2 (97.43), B39 (700.486), B58 (351.8), B62 (479.036) | 4.884 | 0.62 | NA | NT | 0.19305 | |
| ISYAGAYSY | 4 | A1 (766.56), A3 (404.38), B58 (40.114), B62 (59.841) | 5.841 | 0.80 | NA | NT | 0.00822 | |
| SVTIADPGY | 4 | A1 (112.5), A26 (436.77), B58 (385.76), B62 (112.78) | 3.538 | 1.07 | NA | NT | 0.01912 | |
| ALQEVVKAL | 4 | A2 (195.934), B7 (299.5), B8 (307.99), B62 (401.678) | 3.634 | 0.52 | NA | NT | 0.01693 | |
| TMKKIYPAL | 5 | A2 (110.358), A24 (401.48), B8 (119), B39 (103.48), B62 (615.176) | 4.943 | 0.52 | NA | NT | 0.14738 | |
| MVYVITVKY | 4 | A3 (143.81), A26 (636.223), B58 (311.386), B62 (402.71) | 4.385 | 0.91 | NA | NT | 0.11532 | |
| FLFATVPIY | 5 | A3 (509.09), A26 (488.03), B39 (266.701), B58 (266.701), B62 (46.45) | 6.127 | 0.56 | NA | NT | 0.22243 | |
| ORF4b | HSPGKNLRY | 5 | A1 (491.53), A3 (430.28), A26 (220.95), B58 (274.79), B62 (410.42) | 5.421 | 0.43 | NA | NT | 0.16471 |
| SVVTQPTHY | 4 | A1 (532.9), A3 (344.9), A26 (126), B62 (128.60) | 5.572 | 0.53 | NA | NT | 0.01912 | |
| Coronavirus endopeptidase C30 | ASFSVLACY | 5 | A1 (114.33), A3 (91.58), A26 (339.2), B58 (300.82), B62 (127.94) | 5.841 | 0.62 | NA | NT | 0.09719 |
AT, Antigenicity score (HLA supertype with binding affinity scores ≥ 0.75 were taken); NA, Non-allergen; NT, Non-toxic.