Table 4.
Protein | MHCII Epitopes | No. of HLA alleles | HLA alleles/IC50 value (nM) | AT | Allerg. | Tox. |
---|---|---|---|---|---|---|
M | LPNEITVAKPNVLIA | 3 | DRB1*0101/(10.96), DRB1*0701/(27.53), DRB1*1501/(111.34) | 0.41 | NA | NT |
LIALKMVKRQSYGTN | 3 | DRB1*0301/(180.49), DRB1*1101/(46.15), DRB1*1301/(17.3) | 1.00 | NA | NT | |
N | TKSFNMVQAFGLRGA | 3 | DRB1*0101/(3.56), DRB1*0701/(7.18), DRB1*1501/(31.54) | 0.82 | NA | NT |
PKVITKKDAAAAKNK | 6 | DRB1*0101/(213.03), DRB1*0401/(116.37), DRB1*0801/(105.17), DRB1*1101/(77.54), DRB1*1301/(212.59), DRB1*1501/(115.52) | 0.75 | NA | NT | |
SGAIKLDPKNPNYNK | 4 | DRB1*0301/(96.66), DRB1*0801/(177.66), DRB1*1101/(69.44), DRB1*1301/(79.35) | 1.11 | NA | NT | |
PRWYFYYTGTGPEAA | 4 | DRB1*1501/(356.18), DRB1*0401/(155.66), DRB1*0701/(90.79), DRB1*0801/(233.88) | 0.76 | NA | NT | |
S | STSYYSAKPVGAYYE | 5 | DRB1*0101/(6.26), DRB1*0401/(52.57), DRB1*0701/(88.17), DRB1*0801/(274.17), DRB1*1101/(96.90) | 0.43 | NA | NT |
PEPITTLNTRYVAPQ | 5 | DRB1*0101/(36.29), DRB1*0401/(514.57), DRB1*0701/(422.39), DRB1*1301/(126.66), DRB1*1501/(119.58) | 0.82 | NA | NT | |
ISYDIYGITGTGVFQ | 3 | DRB1*0101/(19.77), DRB1*0701/(56.03), DRB1*1501/(155.33) | 0.67 | NA | NT | |
YVAGYKVLPPLMDVN | 3 | DRB1*0101/(18.05), DRB1*0401/(81.41), DRB1*1101/(88.74) | 0.48 | NA | NT | |
GTQYVYSASNHKSTA | 4 | DRB1*0101/(34.89), DRB1*0401/(96.98), DRB1*0701/(153.93), DRB1*1101/(156.61) | 0.45 | NA | NT | |
TQYVYSASNHKSTAN | 3 | DRB1*0101/(34.76), DRB1*0401/(96.43), DRB1*1501/(225.61) | 0.42 | NA | NT | |
IIGFHSDDGNYYCVA | 3 | DRB1*0301/(112.99), DRB1*0401/(258.69), DRB1*1101/(493.8) | 0.46 | NA | NT | |
ORF4a | TAKYTPAPGTSLHPV | 3 | DRB1*0101/(17.07), DRB1*0401/(109.35), DRB1*0701/(448.9) | 0.71 | NA | NT |
ORF4b | ARDISPIAVFLRNVR | 3 | DRB1*0301/(28.77), DRB1*1301/(368.61), DRB1*1501/(91.19) | 1.04 | NA | NT |
ORF5b | STVFVPATRDSVPLH | 3 | DRB1*0301/(436.22), DRB1*0801/(334.17), DRB1*1101/(41.04) | 0.59 | NA | NT |
PLPro | SPDFVAFNVFHGMET | 6 | DRB1*0101/(42.9), DRB1*0401/(84.87), DRB1*0701/(137.13), DRB1*0801/(194.89), DRB1*1101/(173.16), DRB1*1501/(52.95) | 0.71 | NA | NT |
FRTVVLNNKNSYRSQ | 3 | DRB1*0301/(65.99), DRB1*1101/(180.05), DRB1*1501/(21.08) | 0.83 | NA | NT | |
NSP10 | KGKFVQIPSQCTRDP | 5 | DRB1*0101/(7.91), DRB1*0401/(34.54), DRB1*070I/(61.37), DRB1*0801/(191.62), DRB1*1101/(88.24) | 0.57 | NA | NT |
GTGIAISVKPESTAD | 4 | DRB1*0301/(102.9), DRB1*0801/(75.29), DRB1*1101/(350.6), DRB1*1301/(350.6), | 1.21 | NA | NT | |
ADP Binding module |
SKCYRAMNAYPLVVT | 3 | DRB1*0101/(2.95), DRB1*0701/(12.61), DRB1*1501/(30.76) | 0.70 | NA | NT |
AKNILHVVGPDARAK | 3 | DRB1*0101/(12.5), DRB1*0301/(897.1), DRB1*1501/(65.47) | 0.66 | NA | NT | |
CoV endopeptidase C30 | QQLYTGFQGKQILGS | 3 | DRB1*0101/(51.5), DRB1*0701/(118.1), DRB1*1501/(220.03) | 0.41 | NA | NT |
TGTFTVIMRPNYTIK | 3 | DRB1*0801/(72.77), DRB1*1101/(22.62), DRB1*1301/(25.63) | 0.77 | NA | NT |
Predicted IC50 value by the IEDB tool (SMM method) is shown in parenthesis for each epitope with the respective HLA allele. AT, Antigenicity score; Allerg, Allergenicity; Tox, Toxicity (HLA allele with binding affinity scores≥ 0.75 were taken); NA, Non-antigenic; NT, Non-toxic. * is a part of nomenclatures indicating the method is molecular typing.