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. 2022 Aug 3;13:956776. doi: 10.3389/fimmu.2022.956776

Table 4.

List of prioritized MHC-II (HTL) binding epitopes by NetMHCIIpan.

Protein MHCII Epitopes No. of HLA alleles HLA alleles/IC50 value (nM) AT Allerg. Tox.
M LPNEITVAKPNVLIA 3 DRB1*0101/(10.96), DRB1*0701/(27.53), DRB1*1501/(111.34) 0.41 NA NT
LIALKMVKRQSYGTN 3 DRB1*0301/(180.49), DRB1*1101/(46.15), DRB1*1301/(17.3) 1.00 NA NT
N TKSFNMVQAFGLRGA 3 DRB1*0101/(3.56), DRB1*0701/(7.18), DRB1*1501/(31.54) 0.82 NA NT
PKVITKKDAAAAKNK 6 DRB1*0101/(213.03), DRB1*0401/(116.37), DRB1*0801/(105.17), DRB1*1101/(77.54), DRB1*1301/(212.59), DRB1*1501/(115.52) 0.75 NA NT
SGAIKLDPKNPNYNK 4 DRB1*0301/(96.66), DRB1*0801/(177.66), DRB1*1101/(69.44), DRB1*1301/(79.35) 1.11 NA NT
PRWYFYYTGTGPEAA 4 DRB1*1501/(356.18), DRB1*0401/(155.66), DRB1*0701/(90.79), DRB1*0801/(233.88) 0.76 NA NT
S STSYYSAKPVGAYYE 5 DRB1*0101/(6.26), DRB1*0401/(52.57), DRB1*0701/(88.17), DRB1*0801/(274.17), DRB1*1101/(96.90) 0.43 NA NT
PEPITTLNTRYVAPQ 5 DRB1*0101/(36.29), DRB1*0401/(514.57), DRB1*0701/(422.39), DRB1*1301/(126.66), DRB1*1501/(119.58) 0.82 NA NT
ISYDIYGITGTGVFQ 3 DRB1*0101/(19.77), DRB1*0701/(56.03), DRB1*1501/(155.33) 0.67 NA NT
YVAGYKVLPPLMDVN 3 DRB1*0101/(18.05), DRB1*0401/(81.41), DRB1*1101/(88.74) 0.48 NA NT
GTQYVYSASNHKSTA 4 DRB1*0101/(34.89), DRB1*0401/(96.98), DRB1*0701/(153.93), DRB1*1101/(156.61) 0.45 NA NT
TQYVYSASNHKSTAN 3 DRB1*0101/(34.76), DRB1*0401/(96.43), DRB1*1501/(225.61) 0.42 NA NT
IIGFHSDDGNYYCVA 3 DRB1*0301/(112.99), DRB1*0401/(258.69), DRB1*1101/(493.8) 0.46 NA NT
ORF4a TAKYTPAPGTSLHPV 3 DRB1*0101/(17.07), DRB1*0401/(109.35), DRB1*0701/(448.9) 0.71 NA NT
ORF4b ARDISPIAVFLRNVR 3 DRB1*0301/(28.77), DRB1*1301/(368.61), DRB1*1501/(91.19) 1.04 NA NT
ORF5b STVFVPATRDSVPLH 3 DRB1*0301/(436.22), DRB1*0801/(334.17), DRB1*1101/(41.04) 0.59 NA NT
PLPro SPDFVAFNVFHGMET 6 DRB1*0101/(42.9), DRB1*0401/(84.87), DRB1*0701/(137.13), DRB1*0801/(194.89), DRB1*1101/(173.16), DRB1*1501/(52.95) 0.71 NA NT
FRTVVLNNKNSYRSQ 3 DRB1*0301/(65.99), DRB1*1101/(180.05), DRB1*1501/(21.08) 0.83 NA NT
NSP10 KGKFVQIPSQCTRDP 5 DRB1*0101/(7.91), DRB1*0401/(34.54), DRB1*070I/(61.37), DRB1*0801/(191.62), DRB1*1101/(88.24) 0.57 NA NT
GTGIAISVKPESTAD 4 DRB1*0301/(102.9), DRB1*0801/(75.29), DRB1*1101/(350.6), DRB1*1301/(350.6), 1.21 NA NT
ADP
Binding module
SKCYRAMNAYPLVVT 3 DRB1*0101/(2.95), DRB1*0701/(12.61), DRB1*1501/(30.76) 0.70 NA NT
AKNILHVVGPDARAK 3 DRB1*0101/(12.5), DRB1*0301/(897.1), DRB1*1501/(65.47) 0.66 NA NT
CoV endopeptidase C30 QQLYTGFQGKQILGS 3 DRB1*0101/(51.5), DRB1*0701/(118.1), DRB1*1501/(220.03) 0.41 NA NT
TGTFTVIMRPNYTIK 3 DRB1*0801/(72.77), DRB1*1101/(22.62), DRB1*1301/(25.63) 0.77 NA NT

Predicted IC50 value by the IEDB tool (SMM method) is shown in parenthesis for each epitope with the respective HLA allele. AT, Antigenicity score; Allerg, Allergenicity; Tox, Toxicity (HLA allele with binding affinity scores≥ 0.75 were taken); NA, Non-antigenic; NT, Non-toxic. * is a part of nomenclatures indicating the method is molecular typing.