Table 2.
Genetic diversity parameters of the newly developed 33 EST-SSR markers across the 46 Monochasma savatieri individuals
| Primer name | Primer sequence (5′-3′) | SSR motif | Na | MAF | GD | PIC |
|---|---|---|---|---|---|---|
| LRC-19-1 | F:ATACAGTTCGCCGAGCAATC | (CT)12(CA)7 | 4.000 | 0.478 | 0.606 | 0.526 |
| R:TGTGACAGAGCAAGCCAAAC | ||||||
| LRC-38-2 | F:TATTGCGTTCTTTGCTTCCA | (T)17 | 8.000 | 0.261 | 0.817 | 0.791 |
| R:CGACCATCACCTCTAGCTCC | ||||||
| LRC-491-1 | F:AGTTAATGATGCCGATTGCC | (GA)10 | 5.000 | 0.435 | 0.704 | 0.659 |
| R:TAATCTCCTCGCCAAATTGC | ||||||
| LRC-955-2 | F:AGATGCGGTAAACCACAAGG | (T)10 | 5.000 | 0.435 | 0.721 | 0.682 |
| R:AAGAGGGCATTGGCTTTTCT | ||||||
| LRC-1030-1 | F:AAGCTCTGTAATGGCGTTCG | (CT)9 | 6.000 | 0.522 | 0.672 | 0.640 |
| R:TGAATATGCGCTTCGATTGT | ||||||
| LRC-1279-1 | F:TAACCAGAATCCACGTGTCG | (T)10 | 7.000 | 0.565 | 0.635 | 0.605 |
| R:AAGGTCCCATAAGCATCAGC | ||||||
| LRC-3853-1 | F:CTCGCACACTATCTTCCACG | (GGCA)6 | 7.000 | 0.304 | 0.796 | 0.767 |
| R:AGGGATGATGGATACGGATG | ||||||
| LRC-4136-1 | F:TCGCCATAACTCCGTCTTCT | (AG)6 | 6.000 | 0.565 | 0.636 | 0.606 |
| R:GCGAAAGCTATGCCGACTAC | ||||||
| LRC-4412-1 | F:TCCAGTTTTCCATCACCACA | (A)20 | 4.000 | 0.543 | 0.609 | 0.549 |
| R:GCGTCGTTGTAATCATGGTG | ||||||
| LRC-6564-1 | F:ATTCAGGCTTCAGCAGCAAC | (AG)16 | 10.000 | 0.130 | 0.894 | 0.884 |
| R:GTCGTTCAGCAGTGGGATCT | ||||||
| LRC-8962-2 | F:AGAACAAGTCGTCGGAGCAT | (T)21 | 4.000 | 0.489 | 0.619 | 0.547 |
| R:TGCCTTCCCTTGACACTACA | ||||||
| LRC-9052-1 | F:AGATCGATGTGCTCAGCCTT | (GC)7 | 4.000 | 0.413 | 0.680 | 0.621 |
| R:GTCTCAACAAGCGACGTTCA | ||||||
| LRC-10353-1 | F:CGATCTGCTCACAGAACCAA | (CGG)5 | 7.000 | 0.228 | 0.813 | 0.786 |
| R:CAATCGCTGCAGATCGTTTA | ||||||
| LRC-18371-1 | F:TTGACAAGGCATGCAAATTC | (TTC)5 T(TA)14 | 6.000 | 0.435 | 0.693 | 0.641 |
| R:TGAGTTCAACCACTTGGGAG | ||||||
| LRC-18468-1 | F:CCACACGCAGTATTTGGTGA | (CT)6 | 6.000 | 0.457 | 0.702 | 0.660 |
| R:TGCTCGTAATGTGGCTCAAG | ||||||
| LRC-18556-1 | F:TTGCAGAAGAATTGCATTGG | (G)10 | 7.000 | 0.435 | 0.712 | 0.671 |
| R:CACACGCTGCAACTGGTATT | ||||||
| LRC-19013-3 | F:TATTTCACTGAGGTGCGAGC | (ATGT)9 | 11.000 | 0.304 | 0.840 | 0.823 |
| R:TAACGACCACCATTAACGCA | ||||||
| LRC-21021-1 | F:TACACACAATGCATGCCTCA | (C)12 | 8.000 | 0.283 | 0.818 | 0.794 |
| R:GCATGATCCGAAGAGGAGAA | ||||||
| LRC-22140-1 | F:AGTTTGCCGACTCAGAAGGA | (G)14 | 7.000 | 0.500 | 0.656 | 0.606 |
| R:CCTTTGCAGATTTAGAGCCG | ||||||
| LRC-24756-2 | F:TCTCTGCATGCATCCACTTC | (A)11 | 7.000 | 0.391 | 0.758 | 0.726 |
| R:GGCGACGTAGTCATGGAGTT | ||||||
| LRC-25132-1 | F:AGGCGAGTTGGTTTCAGCTA | (ATT)16 | 8.000 | 0.261 | 0.833 | 0.812 |
| R:GAAACATGCGTTTGGTGTTG | ||||||
| LRC-28075-1 | F:CTTTCGCCGTTCAAGTTTTC | (T)13 | 6.000 | 0.391 | 0.753 | 0.719 |
| R:CTCGTTCTTGAGCATGTGGA | ||||||
| LRC-34475-2 | F:TTGGTGCACCTGCTATGTTT | (AT)18 | 7.000 | 0.283 | 0.796 | 0.766 |
| R:CAGAGTGAAACCACAGCCAA | ||||||
| LRC-35320-1 | F:GATTGGCCTCACTTGGTCTC | (AAATT)5 | 8.000 | 0.283 | 0.823 | 0.800 |
| R:TTGCAATGCCATGAACAAAC | ||||||
| LRC-36979-3 | F:TCCATGATTGAAGCTTCTCG | (TG)15 | 10.000 | 0.283 | 0.852 | 0.837 |
| R:TGCAACAAGGAAGAGCAATG | ||||||
| LRC-43046-1 | F:ATTCTCCGTTGGGAGGTTCT | (TA)7 | 8.000 | 0.435 | 0.737 | 0.706 |
| R:AGGAACCAGCTGCACAATCT | ||||||
| LRC-50242-1 | F:TGGAGATTACTTCGCAGCCT | (GA)11 | 4.000 | 0.457 | 0.651 | 0.584 |
| R:CCGATGGTGATTATTGGACC | ||||||
| LRC-55216-1 | F:CTGCAAGTGACATGATGGCT | (CAT)5 | 4.000 | 0.652 | 0.519 | 0.470 |
| R:GCGGAATTGACTGTGAACCT | ||||||
| LRC-55844-1 | F:ATACTCGTTCACCCAATCGC | (T)10C(CT)9 | 6.000 | 0.446 | 0.729 | 0.697 |
| R:TAGCTCCACGATGAAAGGCT | ||||||
| LRC-60747-1 | F:GAAGTGGTGGCACTGAAGGT | (TA)22 | 3.000 | 0.924 | 0.143 | 0.138 |
| R:GCCACTGCCTCTTCTCAGAC | ||||||
| LRC-63796-1 | F:CCATTTGCACTCCGGATACT | (ATTG)5 | 5.000 | 0.478 | 0.689 | 0.647 |
| R:ATCTCCGCCACTGATGTAGG | ||||||
| LRC-68008-1 | F:GGAAACCAACCTGTGCCTTA | (AT)7 | 6.000 | 0.457 | 0.709 | 0.671 |
| R:GGTTTACGGCGAAATCTTGA | ||||||
| LRC-68284-2 | F:CGGACTTCCAATTTCGCTTA | (TTTA)5 | 4.000 | 0.478 | 0.668 | 0.615 |
| R:CCACATAGCTTTACCGGCAT | ||||||
| Mean | 6.303 | 0.424 | 0.706 | 0.668 | ||