Figure 4. Endothelial migration induced by NIR-II exposure.
Cultured HUVECs were serum-starved and treated with 1064 or 1270 nm NIR-II laser at an irradiance of 10 mW/cm2 for 10 min. A, Representative images of scratch assays immediately after the scratches and 12 h after treatment. Scale bars = 500 μm. B, Quantitative analysis of the migration area. Results are expressed as the percentage of wound closure in each experimental group. Results were pooled from two independent experiments. n = 5–6 cell preparations for each group. Error bars denote SEM. A P value less than 0.05 was considered significant: * P < 0.05 by one-way ANOVA followed by Tukey’s multiple comparison test.