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. 1999 Jun;181(11):3582–3586. doi: 10.1128/jb.181.11.3582-3586.1999

TABLE 1.

Quantitation of GltX activity in E. coli JP1449

Plasmida Temp (°C)b GltX sp act (nmol/min/mg of protein)c Relative activity (%)d
None 30 1.15 ± 0.28 100
None 42 0.18 ± 0.06 15.6
pUCP18 30 0.99 ± 0.13 86.1
pUCP18 42 0.17 ± 0.05 14.8
pSal6F 30 7.57 ± 0.01 673.9
pSal6F 42 7.77 ± 0.37 675.6
a

Cell extracts were made from E. coli JP1449 containing the indicated plasmids. 

b

Reaction incubation temperature. 

c

Results are averages of five experiments ± standard deviations. 

d

Activity compared to E. coli JP1449 cell extracts assayed at 30°C.