TABLE 1.
Plasmida | Temp (°C)b | GltX sp act (nmol/min/mg of protein)c | Relative activity (%)d |
---|---|---|---|
None | 30 | 1.15 ± 0.28 | 100 |
None | 42 | 0.18 ± 0.06 | 15.6 |
pUCP18 | 30 | 0.99 ± 0.13 | 86.1 |
pUCP18 | 42 | 0.17 ± 0.05 | 14.8 |
pSal6F | 30 | 7.57 ± 0.01 | 673.9 |
pSal6F | 42 | 7.77 ± 0.37 | 675.6 |
Cell extracts were made from E. coli JP1449 containing the indicated plasmids.
Reaction incubation temperature.
Results are averages of five experiments ± standard deviations.
Activity compared to E. coli JP1449 cell extracts assayed at 30°C.