TABLE 2.
Effects of o-phenanthroline on GltX activity
| Concn (mM)a | GltX sp act (nmol/min/mg of protein)b | Relative activity (%)c |
|---|---|---|
| 0 | 8.58 ± 0.68 | 100.0 |
| 1 | 0.79 ± 0.49 | 9.3 |
| 10 | 0.10 ± 0.11 | 1.2 |
a
GltX reactions were performed at 42°C with 20 μg of E. coli JP1449(pSal6F) extracts after preincubation of cell proteins with the indicated concentrations of o-phenanthroline for 10 min.
b
Results are averages of five experiments ± standard deviations.
c
Activity compared to assays with no added o-phenanthroline.