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. 2022 Aug 3;3:952753. doi: 10.3389/falgy.2022.952753

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Copyright © 2022 Kaplan, Joseph and Ghebrehiwet.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Localization of gC1qR on HUVEC's by immunochemical staining. Monolayer cultures of HUVECs on slides are fixed using 4% formaldehyde. The cells were first probed with rabbit anti gC1qR antibody and subsequently with horse radish -labeled goat anti-rabbit IgG. The gC1qR was visualized by 3,3-diaminobenzidine. Preimmune rabbit IgG staining showed no signal (A) and the anti-gC1qR antibody stained at the cell surface in non-permobilized cells (B) while in permimobilized cells (C), the perinuclear and nuclear regions were prominently stained. In D, the cells were treated as in (B) but the antibody was pretreated with excess recombinant gC1qR.