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. 2022 Jul 14;149(14):dev200154. doi: 10.1242/dev.200154

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© 2022. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 6. — Repression of epidermal Wnt signaling causes ectopic localization of MCs to interscale IFE. (A) Immunostaining of tail epidermis wholemounts of 3-month-old control and K14ΔNLef1 mice for langerin, Trp2 and K31. Representative for n=6. (B) γδTCR and langerin immunostaining of tail epidermis wholemounts from 3-month-old control and K14ΔNLef1 mice. Representative for n=4. (C) Illustration of one scale:interscale unit with corresponding categorization into scale center, scale periphery and interscale IFE for analysis of MC distribution in K14ΔNLef1 mice as in D, G and H. (D) Quantification of A: MC distribution per scale:interscale unit (% of MCs) in control and K14ΔNLef1 mice. MC numbers per compartment were normalized to the total number of the scale:interscale unit. n=6; ****P<0.0001 (scale center, Ctrl versus K14ΔNLef1; interscale IFE, Ctrl versus K14ΔNLef1); ns, P=0.9995 (scale periphery, Ctrl versus K14ΔNLef1); mean±s.d.; one-way ANOVA/Tukey's multiple comparisons test. (E) Trp2 immunostaining of ear epidermis wholemounts of 3-month-old control and K14ΔNLef1 mice. Nuclei were counterstained using DAPI. Representative for n=5. (F) Quantification of E: MC density (MC number/mm²) in ear epidermis. n=5; **P=0.0079; mean±s.d.; Mann–Whitney test. (G) Quantification of A: LC distribution per scale:interscale unit (% of LCs) in control and K14ΔNLef1 mice. LC numbers per compartment were normalized to the total number of the scale:interscale unit. n=6; ns, P=0.9991 (scale center, Ctrl versus K14ΔNLef1); ns, P=0.1341 (scale periphery, Ctrl versus K14ΔNLef1); ns, P=0.2596 (interscale IFE, Ctrl versus K14ΔNLef1); mean±s.d.; one-way ANOVA/Tukey's multiple comparisons test. (H) Quantification of B: DETC distribution per scale:interscale unit (% of DETCs) in control and K14ΔNLef1 mice. DETC numbers per compartment were normalized to the total number of the scale:interscale unit. n=6; ns, P>0.9999 (scale center, Ctrl versus K14ΔNLef1); *P=0.0163 (scale periphery, Ctrl versus K14ΔNLef1); *P=0.0166 (interscale IFE, Ctrl versus K14ΔNLef1); mean±s.d.; one-way ANOVA/Tukey's multiple comparisons test. is, interscale; s, scale. Scale bars: 100 µm (A); 250 µm (B,E).