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. 2022 Jul 22;149(14):dev200533. doi: 10.1242/dev.200533

Fig. 8.

Fig. 8.

Alterations of Alx family genes in Wls-cKO midfacial primordia at E11.5. (A-F) Front views of whole-mount in situ hybridization of control and Wls-cKO facial primordia showing altered expression patterns of Alx1, Alx3 and Alx4. Arrows indicate diminished expression, and arrowheads indicate upregulated expression in the mutant primordia. (G,I,K) RT-qPCR validation of the indicated genes in WT and Wls-cKO embryos (n=6 per group). *P<0.05; ***P<0.001; ns, no statistical significance (t-test). (H,J,L) Violin plots of scRNA-seq showing downregulated expression of Alx1 in m9 and concurrent upregulation in m5 of the mutant MNPs and Alx3 upregulation in m2 and m7 of the mutant LNPs. Alx4 alterations were not detected by scRNA-seq. Dashed boxes indicate cell clusters with altered gene expression in the mutants (black, upregulated; red, downregulated). Asterisks indicate statistical significance (P_adj<0.05; bimod test). lnp, lateral nasal prominence; man, mandibular prominence; mnp, medial nasal prominence; mxp, maxillary prominence.