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Bmp7 in situ hybridization and RT-qPCR. (A) In situ hybridization of controls (Aa-Ad) and Bmp7 mutants (Ae-Ah). Embryonic or postnatal day indicated at top of each column. The arrows delimit the region of Bmp7 expression in maturing nephrons outside the nephrogenic zone. Scale bar: 100 µm. (B) RT-qPCR for Bmp7 in P2 kidneys. Data are mean±s.d. ****P<0.0001 (unpaired two-tailed t-test). Three biological replicate kidneys were examined at each time point.
