Table 4.

Sub-analysis of changes in CTL, T_reg, and CTL/T_reg ratio in each parameter.

Parameter	Patient no.	CTL			Treg			CTL/Treg ratio
		Baseline	After treatment	p Value	Baseline	After treatment	p Value	Baseline	After treatment	p Value
Age (years)	8
≦60	11	27.6 ± 2.7	31.2 ± 2.9	.10	2.6 ± 0.5	2.4 ± 0.6	.52	15.9 ± 5.3	19.2 ± 4.2	.33
>60		27.4 ± 3.2	29.5 ± 3.9	.24	1.8 ± 0.3	1.6 ± 0.2	.43	20.9 ± 5.2	23.3 ± 5.5	.14
Gender
Male	10	30.8 ± 2.9	34.7 ± 3.4	.10	1.8 ± 0.3	1.5 ± 0.2	.17	23.4 ± 5.5	28.2 ± 5.7	<.02
Female	9	23.9 ± 2.8	25.2 ± 3.1	.22	2.6 ± 0.5	2.5 ± 0.6	.78	13.7 ± 4.4	14.3 ± 2.8	.82
Primary diagnosis
Colorectal	8	22.6 ± 2.5	24.5 ± 3.5	.43	2.4 ± 0.3	2.2 ± 0.4	.54	11.6 ± 3.4	15.7 ± 4.3	.06
Soft tissue	8	28.5 ± 2.4	32.1 ± 2.9	.12	2.1 ± 0.5	2.0 ± 0.5	.75	19.4 ± 4.5	20.3 ± 2.8	.79
Lung	2	…	…	…	…	…	…	…	…	…
Gynecologic	1	…	…	…	…	…	…	…	…	…
Target tumor
Primary	3	27.5 ± 2.1	30.2 ± 2.5	.21	2.1 ± 0.3	1.9 ± 0.3	.11	18.8 ± 3.7	21.6 ± 3.6	.54
Metastasis	16	25.9 ± 2.3	28.5 ± 2.8	.09	2.1 ± 0.2	1.8 ± 0.2	.21	16.7 ± 3.7	20.7 ± 3.9	<.02
Ablation site
Lung	7	27.9 ± 4.6	32.0 ± 5.3	.07	1.8 ± 0.3	1.9 ± 0.5	.71	23.2 ± 7.2	28.1 ± 7.9	<.05
Soft tissue	5	26.7 ± 2.7	31.1 ± 3.6	.13	3.5 ± 0.5	2.6 ± 0.8	.06	7.9 ± 0.8	14.4 ± 2.2	<.03
Liver	4	25.7 ± 2.2	29.4 ± 5.8	.51	1.9 ± 0.2	1.7 ± 0.3	.60	13.9 ± 2.0	17.8 ± 4.4	.26
Bone	3	31.3 ± 3.9	30.2 ± 4.0	.20	1.0 ± 0.2	1.3 ± 0.2	.11	32.0 ± 6.4	24.4 ± 2.5	.19
Target tumor number
Single	16	27.1 ± 2.1	30.6 ± 2.9	.05	2.2 ± 0.4	2.0 ± 0.4	.39	18.3 ± 3.8	20.3 ± 3.5	.35
2 or 3	3	28.4 ± 5.4	29.4 ± 5.3	.59	2.1 ± 0.3	1.8 ± 0.6	.59	19.9 ± 8.9	24.5 ± 8.9	.12
Maximum diameter of target tumor (cm)
≦2	13	24.4 ± 1.9	28.4 ± 2.8	.35	2.1 ± 0.3	1.9 ± 0.3	.82	15.5 ± 3.1	19.5 ± 3.4	.06
>2	6	34.1 ± 4.6	34.2 ± 5.2	.94	2.3 ± 0.7	2.0 ± 0.7	.44	25.9 ± 9.4	26.1 ± 9.0	.96
Ablation technique
Heat-based ablation	14	26.6 ± 2.7	29.0 ± 3.0	.13	2.1 ± 0.3	1.9 ± 0.3	.39	18.0 ± 4.4	21.6 ± 4.7	<.02
Cryoablation	5	29.9 ± 3.2	33.7 ± 4.6	.24	2.3 ± 0.8	2.1 ± 0.9	.66	21.0 ± 7.0	21.5 ± 4.3	.92
Ablative zone area (cm2)
≦10	11	25.5 ± 2.1	29.3 ± 3.3	.08	2.4 ± 0.4	2.2 ± 0.4	.32	14.5 ± 3.1	17.4 ± 3.3	.09
>10	8	30.2 ± 4.1	31.5 ± 4.1	.38	4.5 ± 0.5	3.9 ± 0.6	.65	24.8 ± 7.3	27.5 ± 7.0	.43

Data are presented as mean and standard error (SE).

CTL: cytotoxic T-lymphocyte; T_reg: regulatory T-cell.